Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.

ABSTRACT:

Introduction

Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations.

Methods

We evaluated the performance of two SFC and MC panels, which contained 21 common markers, for the identification and subsetting of blood IMC populations. Based on unsupervised clustering analysis, we systematically identified 24 leukocyte popu

SUBMITTER: van der Pan K 

PROVIDER: S-EPMC10235610 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature