Spatial distribution of tumor-infiltrating T cells indicated immune response status under chemoradiotherapy plus PD-1 blockade in esophageal cancer.

ABSTRACT:

Background

The spatial distribution of tumor-infiltrating T cells and its dynamics during chemoradiotherapy combined with PD-1 blockade is little known in esophageal squamous cell carcinoma (ESCC).

Methods

We applied the multiplex immunofluorescence method to identify T cells (CD4⁺, CD8⁺ T cells, and their PD-1^- or PD-1⁺ subsets) and myeloid-derived cells (CD11c⁺ dendritic cells, CD68⁺ macrophages, and their PD-L1⁺ subpopulations) in paired tumor biopsies (n = 36) collected at baseline and during combination (40 Gy of radiation) from a phase Ib trial (NCT03671265) of ESCC patients treated with first-line chemoradiotherapy plus anti-PD-1 antibody camrelizumab. We used the FoundationOne CDx ass

SUBMITTER: Yan C 

PROVIDER: S-EPMC10235618 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature