Developmental patterning of peptide transcription in the central circadian clock in both sexes.

ABSTRACT:

Introduction

Neuropeptide signaling modulates the function of central clock neurons in the suprachiasmatic nucleus (SCN) during development and adulthood. Arginine vasopressin (AVP) and vasoactive intestinal peptide (VIP) are expressed early in SCN development, but the precise timing of transcriptional onset has been difficult to establish due to age-related changes in the rhythmic expression of each peptide.

Methods

To provide insight into spatial patterning of peptide transcription during SCN development, we used a transgenic approach to define the onset of Avp and Vip transcription. Avp-Cre or Vip-Cre males were crossed to Ai9^+/+ females, producing offspring in which the fluorescent protein tdTomato (tdT) is expressed at the onset of

SUBMITTER: Carmona-Alcocer V 

PROVIDER: S-EPMC10235759 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature