Dimer-assisted mechanism of (un)saturated fatty acid decarboxylation for alkene production.

ABSTRACT: The enzymatic decarboxylation of fatty acids (FAs) represents an advance toward the development of biological routes to produce drop-in hydrocarbons. The current mechanism for the P450-catalyzed decarboxylation has been largely established from the bacterial cytochrome P450 OleT_JE. Herein, we describe OleTP_RN, a poly-unsaturated alkene-producing decarboxylase that outrivals the functional properties of the model enzyme and exploits a distinct molecular mechanism for substrate binding and chemoselectivity. In addition to the high conversion rates into alkenes from a broad range of saturated FAs without dependence on high salt concentrations, OleTP_RN can also efficiently produce alkenes from unsaturated (oleic and linoleic) acids, the most abundant FAs found

SUBMITTER: Rade LL 

PROVIDER: S-EPMC10235961 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature