CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products.

ABSTRACT: Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagenesis to confirm candidate genes identified using map-based methods. Researchers are also developing tools to more effectively apply CRISPR-Cas technologies to maize because successful application of CRISPR-Cas relies on target gene identification, guide RNA development, vector design and construction, CRISPR-Cas reagent delivery to maize tissues, and plant characterization, each contributing unique challenges to CRISPR-Cas efficacy. Recent advances continue to chip away at major barriers that pre

SUBMITTER: Nuccio ML 

PROVIDER: S-EPMC10236071 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature