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Characterization of a Potential Probiotic Lactiplantibacillus plantarum LRCC5310 by Comparative Genomic Analysis and its Vitamin B6 Production Ability.


ABSTRACT: Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain's probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B6 biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917T, L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5'-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B6 supplementation.

SUBMITTER: Lee Y 

PROVIDER: S-EPMC10236179 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Characterization of a Potential Probiotic <i>Lactiplantibacillus plantarum</i> LRCC5310 by Comparative Genomic Analysis and its Vitamin B<sub>6</sub> Production Ability.

Lee Yunjeong Y   Jaikwang Nattira N   Kim Seong Keun SK   Jeong Jiseon J   Sukhoom Ampaitip A   Kim Jong-Hwa JH   Kim Wonyong W  

Journal of microbiology and biotechnology 20230206 5


Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. <i>Lactiplantibacillus plantarum</i> is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of <i>L. plantarum</i> LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biot  ...[more]

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