Fragment-Based Ligand Discovery Using Protein-Observed ¹⁹F NMR: A Second Semester Organic Chemistry CURE Project.

ABSTRACT: Curriculum-based undergraduate research experiences (CUREs) have been shown to increase student retention in STEM fields and are starting to become more widely adopted in chemistry curricula. Here we describe a 10-week CURE that is suitable for a second-semester organic chemistry laboratory course. Students synthesize small molecules and use protein-observed ¹⁹F (PrOF) NMR to assess the small molecule's binding affinity to a target protein. The research project introduced students to multistep organic synthesis, structure-activity relationship studies, quantitative biophysical measurements (measuring K_d from PrOF NMR experiments), and scientific literacy. Docking experiments could be added to help students understand how changes in a ligand structure may affect

SUBMITTER: Bur SK 

PROVIDER: S-EPMC10237086 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature