Protein production from HEK293 cell line-derived stable pools with high protein quality and quantity to support discovery research.

ABSTRACT: Antibody-based therapeutics and recombinant protein reagents are often produced in mammalian expression systems, which provide human-like post-translational modifications. Among the available mammalian cell lines used for recombinant protein expression, Chinese hamster ovary (CHO)-derived suspension cells are generally utilized because they are easy to culture and tend to produce proteins in high yield. However, some proteins purified from CHO cell overexpression suffer from clipping and display undesired non-human post translational modifications (PTMs). In addition, CHO cell lines are often not suitable for producing proteins with many glycosylation motifs for structural biology studies, as N-linked glycosylation of proteins poses challenges for structure determination by X-ray crystallo

SUBMITTER: Sun H 

PROVIDER: S-EPMC10237474 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature