Detection of Canine Parvovirus Type 2 by Designing Multiple Methods and Genetic Characterization in Iran.

ABSTRACT: Canine parvovirus infection is the most highly infectious in dogs younger than six months. Our study aimed to design and optimize an In-house PCR Assay for Rapid Detection of parvovirus type 2 and compares it with REAL-TIME PCR and LAMP Assay and phylogenetic analysis. The virulence gene selected for the categories was vp₂ for CPV-2. PCR products were cloned in pTZ57R/T plasmid for preparation of positive control. Determination of the specificity of primers was done with the negative control virus genomes, and the limit of detection was determined for the Homemade PCR, REAL-TIME PCR, LAMP, and to perform a phylogenetic study using partial vp₂ gene sequences. Added analysis of PCR products using agarose gel electrophoresis for the vp₂ ge

SUBMITTER: Morovvati A 

PROVIDER: S-EPMC10237543 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature