Multiplex Digital Methylation-Specific PCR for Noninvasive Screening of Lung Cancer.

ABSTRACT: There remains tremendous interest in developing liquid biopsy assays for detection of cancer-specific alterations, such as mutations and DNA methylation, in cell-free DNA (cfDNA) obtained through noninvasive blood draws. However, liquid biopsy analysis is often challenging due to exceedingly low fractions of circulating tumor DNA (ctDNA), necessitating the use of extended tumor biomarker panels. While multiplexed PCR strategies provide advantages such as higher throughput, their implementation is often hindered by challenges such as primer-dimers and PCR competition. Alternatively, digital PCR (dPCR) approaches generally offer superior performance, but with constrained multiplexing capability. This paper describes development and validation of the first multiplex digital methylation-specif

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC10238189 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature