Application of MALDI-TOF MS for enumerating bacterial constituents of defined consortia.

ABSTRACT: Characterization of live biotherapeutic product (LBP) batches typically includes a measurement of viability, such as colony forming units (CFU). However, strain-specific CFU enumeration assays can be complicated by the presence of multiple organisms in a single product with similar growth requirements. To overcome specific challenges associated with obtaining strain-specific CFU values from multi-strain mixtures, we developed a method combining mass spectrometry-based colony identification with a traditional CFU assay. This method was assessed using defined consortia made from up to eight bacterial strains. Among four replicate batches of an eight-strain mixture, observed values differed from expected values by less than 0.4 log₁₀ CFU among all strains measured (range of differe

SUBMITTER: Coryell MP 

PROVIDER: S-EPMC10238304 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature