New robust subtilisins from halotolerant and halophilic Bacillaceae.

ABSTRACT: The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465^T and Alkalibacillus haloalkaliphilus DSM 5271^T and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976^T served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned a

SUBMITTER: Falkenberg F 

PROVIDER: S-EPMC10238314 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature