Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells.

ABSTRACT: The mesenchymal cancer phenotype is known to be clinically related to treatment resistance and a poor prognosis. We identified gene signature-based molecular subtypes of gastric cancer (GC, n = 547) based on transcriptome data and validated their prognostic and predictive utility in multiple external cohorts. We subsequently examined their associations with tumor microenvironment (TME) features by employing cellular deconvolution methods and sequencing isolated GC populations. We further performed spatial transcriptomics analysis and immunohistochemistry, demonstrating the presence of GC cells in a partial epithelial-mesenchymal transition state. We performed network and pharmacogenomic database analyses to identify TGF-β signaling as a driver pathway and, thus, a therapeutic target. We fu

SUBMITTER: Jang E 

PROVIDER: S-EPMC10238377 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature