Spatiotemporal transcriptomic atlas reveals the dynamic characteristics and key regulators of planarian regeneration.

ABSTRACT: Whole-body regeneration of planarians is a natural wonder but how it occurs remains elusive. It requires coordinated responses from each cell in the remaining tissue with spatial awareness to regenerate new cells and missing body parts. While previous studies identified new genes essential to regeneration, a more efficient screening approach that can identify regeneration-associated genes in the spatial context is needed. Here, we present a comprehensive three-dimensional spatiotemporal transcriptomic landscape of planarian regeneration. We describe a pluripotent neoblast subtype, and show that depletion of its marker gene makes planarians more susceptible to sub-lethal radiation. Furthermore, we identified spatial gene expression modules essential for tissue development. Functional analys

SUBMITTER: Cui G 

PROVIDER: S-EPMC10238425 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature