Cellular heterogeneity in the 16HBE14o^- airway epithelial line impacts biological readouts.

ABSTRACT: The airway epithelial cell line, 16HBE14o^- , is an important cell model for studying airway disease. 16HBE14o^- cells were originally generated from primary human bronchial epithelial cells by SV40-mediated immortalization, a process that is associated with genomic instability through long-term culture. Here, we explore the heterogeneity of these cells, with respect to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) transcript and protein. We isolate clones of 16HBE14o^- with stably higher and lower levels of CFTR in comparison to bulk 16HBE14o^- , designated CFTR^high and CFTR^low . Detailed characterization of the CFTR locus in these clones by ATAC-seq and 4C-seq showed open chromatin profiles and h

SUBMITTER: Kerschner JL 

PROVIDER: S-EPMC10238858 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature