Formation of protein adducts with Hydroperoxy-PE electrophilic cleavage products during ferroptosis.

ABSTRACT: Ferroptosis is an iron dependent form of cell death, that is triggered by the discoordination of iron, lipids, and thiols. Its unique signature that distinguishes it from other forms of cell death is the formation and accumulation of lipid hydroperoxides, particularly oxidized forms of polyunsaturated phosphatidylethanolamines (PEs), which drives cell death. These readily undergo iron-catalyzed secondary free radical reactions leading to truncated products which retain the signature PE headgroup and which can readily react with nucleophilic moieties in proteins via their truncated electrophilic acyl chains. Using a redox lipidomics approach, we have identified oxidatively-truncated PE species (trPEox) in enzymatic and non-enzymatic model systems. Further, using a model peptide we demonstra

SUBMITTER: Amoscato AA 

PROVIDER: S-EPMC10238881 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature