Longitudinal Immune Cell Profiling in Patients With Early Systemic Lupus Erythematosus.

ABSTRACT:

Objectives

To investigate the immune cell profiles of patients with systemic lupus erythematosus (SLE), and to identify longitudinal changes in those profiles over time.

Methods

We employed mass cytometry with 3 different panels of 38-39 markers (an immunophenotyping panel, a T cell/monocyte panel, and a B cell panel) in cryopreserved peripheral blood mononuclear cells (PBMCs) from 9 patients with early SLE, 15 patients with established SLE, and 14 controls without autoimmune disease. We used machine learning-driven clustering, flow self-organizing maps, and dimensional reduction with t-distributed stochastic neighbor embedding to identify unique cell populations in early SLE and established SLE. We used mass cytometry data of PBMCs from 19 patients with early rheumatoid art

SUBMITTER: Sasaki T 

PROVIDER: S-EPMC10238884 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature