Increased levels of PD1 and glycolysis in CD4⁺ T cells are positively associated with lymph node metastasis in OSCC.

ABSTRACT:

Background

Cervical lymph node metastasis is one of the poorest prognostic factors in oral squamous cell carcinoma (OSCC). Activated immune cells generally have metabolic abnormalities in the tumour microenvironment. However, it is unknown whether abnormal glycolysis in T cells could facilitate metastatic lymph nodes in OSCC patients. The aim of this study was to investigate the effects of immune checkpoints in metastatic lymph nodes and determine the correlation between glycolysis and immune checkpoint expression in CD4⁺ T cells.

Methods

Flow cytometry and immunofluorescence staining were used to analyse the differences in CD4⁺ PD1⁺ T cells between metastatic lymph nodes (LN⁺) and negative lymph nodes (LN^-). RT‒PCR was

SUBMITTER: Wu K 

PROVIDER: S-EPMC10239138 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature