Systematic literature review reveals suboptimal use of chemical probes in cell-based biomedical research.

ABSTRACT: Chemical probes have reached a prominent role in biomedical research, but their impact is governed by experimental design. To gain insight into the use of chemical probes, we conducted a systematic review of 662 publications, understood here as primary research articles, employing eight different chemical probes in cell-based research. We summarised (i) concentration(s) at which chemical probes were used in cell-based assays, (ii) inclusion of structurally matched target-inactive control compounds and (iii) orthogonal chemical probes. Here, we show that only 4% of analysed eligible publications used chemical probes within the recommended concentration range and included inactive compounds as well as orthogonal chemical probes. These findings indicate that the best practice with chemical pr

SUBMITTER: Sterling J 

PROVIDER: S-EPMC10239480 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature