Preventing swarm detection in extracellular vesicle flow cytometry: a clinically applicable procedure.

ABSTRACT:

Background

Flow cytometry is commonly used to detect cell-derived extracellular vesicles in body fluids such as blood plasma. However, continuous and simultaneous illumination of multiple particles at or below the detection limit may result in the detection of a single event. This phenomenon is called swarm detection and leads to incorrect particle concentration measurements. To prevent swarm detection, sample dilution is recommended. Since the concentration of particles differs between plasma samples, finding the optimal sample dilution requires dilution series of all samples, which is unfeasible in clinical routine.

Objectives

Here we developed a practical procedure to find the optimal sample dilution of plasma for extracellular vesicle flow cytometry measurements in clini

SUBMITTER: Buntsma NC 

PROVIDER: S-EPMC10239693 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature