Metagenomic analysis reveals gut plasmids as diagnosis markers for colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) is linked to distinct gut microbiome patterns. The efficacy of gut bacteria as diagnostic biomarkers for CRC has been confirmed. Despite the potential to influence microbiome physiology and evolution, the set of plasmids in the gut microbiome remains understudied.

Methods

We investigated the essential features of gut plasmid using metagenomic data of 1,242 samples from eight distinct geographic cohorts. We identified 198 plasmid-related sequences that differed in abundance between CRC patients and controls and screened 21 markers for the CRC diagnosis model. We utilize these plasmid markers combined with bacteria to construct a random forest classifier model to diagnose CRC.

Results

The plasmid markers were able to distinguish betwee

SUBMITTER: Cai Z 

PROVIDER: S-EPMC10239823 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature