Glabridin inhibited the spread of polymyxin-resistant Enterobacterium carrying ICEMmoMP63.

ABSTRACT:

Introduction

The role of integrative and conjugative elements (ICEs) in antibiotic resistance in Morganella morganii is unknown. This study aimed to determine whether an ICE identified in the M. morganii genome contributed to the polymyxin resistance.

Methods

Whole-genome sequencing was performed followed by bioinformatics analyses to identify ICEs and antibiotic resistance genes. Conjugation assays were performed to analyze the transferability of a discovered ICE. A drug transporter encoded on the ICE was heterogeneously expressed in Escherichia coli, minimum inhibitory concentrations of antibiotics were determined, and a traditional Chinese medicine library was screened for potential efflux pump inhibitors.

Results

An antibiotic resistance-confe

SUBMITTER: Fu J 

PROVIDER: S-EPMC10239875 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature