Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures.

ABSTRACT: Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate environment can result in the rapid conversion of stem cells into progenitors/transient amplifying cells (clonal conversion), with deleterious consequences on the quality of the transplants and their ability to engraft. Here, we demonstrate that cultured human epidermal stem cells respond to a small drop in temperature through thermoTRP channels via mTOR signaling. Exposure of cells to rapamycin or a small drop in temperature induces the nuclear translocation of mTOR with an impact on gene expression. We also demonstrate by single-cell an

SUBMITTER: Nanba D 

PROVIDER: S-EPMC10240198 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature