Characterisation of hepatic lipid signature distributed across the liver zonation using mass spectrometry imaging.

ABSTRACT:

Background & aims

Lipid metabolism plays an important role in liver pathophysiology. The liver lobule asymmetrically distributes oxygen and nutrition, resulting in heterogeneous metabolic functions. Periportal and pericentral hepatocytes have different metabolic functions, which lead to generating liver zonation. We developed spatial metabolic imaging using desorption electrospray ionisation mass spectrometry to investigate lipid distribution across liver zonation with high reproducibility and accuracy.

Methods

Fresh frozen livers from healthy mice with control diet were analysed using desorption electrospray ionisation mass spectrometry imaging. Imaging was performed at 50 μm × 50 μm pixel size. Regions of interest (ROIs) were manually created by co-registering with histolo

SUBMITTER: Seubnooch P 

PROVIDER: S-EPMC10240278 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature