Targeted imaging of very late antigen-4 for noninvasive assessment of lung inflammation-fibrosis axis.

ABSTRACT:

Background

The lack of noninvasive methods for assessment of dysregulated inflammation as a major driver of fibrosis (i.e., inflammation-fibrosis axis) has been a major challenge to precision management of fibrotic lung diseases. Here, we determined the potential of very late antigen-4 (VLA-4)-targeted positron emission tomography (PET) to detect inflammation in a mouse model of bleomycin-induced fibrotic lung injury.

Method

Single time-point and longitudinal VLA-4-targeted PET was performed using a high-affinity peptidomimetic radiotracer, ⁶⁴Cu-LLP2A, at weeks 1, 2, and 4 after bleomycin-induced (2.5 units/kg) lung injury in C57BL/6J mice. The severity of fibrosis was determined by measuring the hydroxyproline content of the lungs and expression of markers of ext

SUBMITTER: Zhu Q 

PROVIDER: S-EPMC10240482 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature