A Conserved Histidine Residue Drives Extein Dependence in an Enhanced Atypically Split Intein.

ABSTRACT: Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and biotechnology to carry out traceless and specific protein ligation. However, the external residues immediately flanking the intein (exteins) can reduce the splicing rate, thereby limiting certain applications of PTS. Splicing by a recently developed intein with atypical split architecture ("Cat") exhibits a stark dependence on the sequence of its N-terminal extein residues. Here, we further developed Cat using error-prone polymerase chain reaction (PCR) and a cell-based selection assay to produce Cat*, which exhibits greatly enhanced PTS activity in the presence of unfavorable N-extein residues. We then applied solution nuclear magnetic resonance spectroscopy and molecular dynamics simulations to e

SUBMITTER: Sekar G 

PROVIDER: S-EPMC10241006 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature