Cellular and molecular profiles of larval and adult Xenopus corneal epithelia resolved at the single-cell level.

ABSTRACT: Corneal Epithelial Stem Cells (CESCs) and their proliferative progeny, the Transit Amplifying Cells (TACs), are responsible for homeostasis and maintaining corneal transparency. Owing to our limited knowledge of cell fates and gene activity within the cornea, the search for unique markers to identify and isolate these cells remains crucial for ocular surface reconstruction. We performed single-cell RNA sequencing of corneal cells from larval and adult stages of Xenopus. Our results indicate that as the cornea develops and matures, there is an increase in cellular diversity, which is accompanied by a substantial shift in transcriptional profile, gene regulatory network and cell-cell communication dynamics. Our data also reveals several novel genes expressed in corneal cells and changes in g

SUBMITTER: Sonam S 

PROVIDER: S-EPMC10241109 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature