Metabolic Labeling-Based Chemoproteomics Establishes Choline Metabolites as Protein Function Modulators.

ABSTRACT: Choline is an essential nutrient for mammalian cells. Our understanding of the cellular functions of choline and its metabolites, independent of their roles as choline lipid metabolism intermediates, remains limited. In addition to fundamental cellular physiology, this knowledge has implications for cancer biology because elevated choline metabolite levels are a hallmark of cancer. Here, we establish a mammalian choline metabolite-interacting proteome by utilizing a photocrosslinkable choline probe. To design this probe, we performed metabolic labeling experiments with structurally diverse choline analogues that resulted in the serendipitous discovery of a choline lipid headgroup remodeling mechanism involving sequential dealkylation and methylation steps. We demonstrate that phosphocholin

SUBMITTER: Dixit A 

PROVIDER: S-EPMC10241378 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature