Integrative profiling of gene expression and chromatin accessibility elucidates specific transcriptional networks in porcine neutrophils.

ABSTRACT: Neutrophils are vital components of the immune system for limiting the invasion and proliferation of pathogens in the body. Surprisingly, the functional annotation of porcine neutrophils is still limited. The transcriptomic and epigenetic assessment of porcine neutrophils from healthy pigs was performed by bulk RNA sequencing and transposase accessible chromatin sequencing (ATAC-seq). First, we sequenced and compared the transcriptome of porcine neutrophils with eight other immune cell transcriptomes to identify a neutrophil-enriched gene list within a detected neutrophil co-expression module. Second, we used ATAC-seq analysis to report for the first time the genome-wide chromatin accessible regions of porcine neutrophils. A combined analysis using both transcriptomic and chromatin accessi

SUBMITTER: Herrera-Uribe J 

PROVIDER: S-EPMC10242145 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature