Dataset Information

Identification and Verification of Key MiRNAs Associated with Intervertebral Disc Degeneration.

ABSTRACT:

Background

Intervertebral Disc Degeneration (IDD) is a heterogeneous spinal disease whose underlying molecular mechanism is unclear.

Objectives

This study aimed to identify, profile, and analyze microRNAs (miRNAs) related to IDD.

Methods

Microarray Gene Expression IDD data (GSE63492) were downloaded from Gene Expression Omnibus datasets. We employed Weighted Gene Co-Expression Network Analysis (WGCNA) to construct a miRNA co-expression network, and the miRNAs related to the IDD stage were detected. The number of differentially expressed miRNAs between normal and degenerated nucleus pulposus tissues was calculated. Twenty-three clinical specimens were used to validate the expression of miRNAs using qRT-PCR.