RegiSTORM: channel registration for multi-color stochastic optical reconstruction microscopy.

ABSTRACT:

Background

Stochastic optical reconstruction microscopy (STORM), a super-resolution microscopy technique based on single-molecule localizations, has become popular to characterize sub-diffraction limit targets. However, due to lengthy image acquisition, STORM recordings are prone to sample drift. Existing cross-correlation or fiducial marker-based algorithms allow correcting the drift within each channel, but misalignment between channels remains due to interchannel drift accumulating during sequential channel acquisition. This is a major drawback in multi-color STORM, a technique of utmost importance for the characterization of various biological interactions.

Results

We developed RegiSTORM, a software for reducing channel misalignment by accurately registering STORM channe

SUBMITTER: Ovrebo O 

PROVIDER: S-EPMC10242778 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature