Single-cell characteristics and malignancy regulation of alpha-fetoprotein-producing gastric cancer.

ABSTRACT:

Objective

To characterize alpha-fetoprotein (AFP)-producing gastric cancer (AFPGC) at the single-cell level and to identify regulatory factors for AFP expression and malignancy.

Methods

ScRNA-seq was performed on two tumors collected from patients with AFPGC. InferCNV and sub-clustering were applied to identify typical AFPGC cells, followed by AddModuleScore, pathway enrichment, Pseudo-time, and Scenic analyses. Data from a gastric cancer (GC) cohort were collected for conjoint analysis. The analytical results were verified by cell experiments and immunohistochemistry.

Results

AFPGC cells are similar to hepatocytes in transcriptome and transcriptional regulation, with kinetic malignancy-related pathways, compared to the common malignant epithelium. In addition, compar

SUBMITTER: Mei Y 

PROVIDER: S-EPMC10242870 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature