Structural basis of dual activation of cell division by the actinobacterial transcription factors WhiA and WhiB.

ABSTRACT: Studies of transcriptional initiation in different bacterial clades reveal diverse molecular mechanisms regulating this first step in gene expression. The WhiA and WhiB factors are both required to express cell division genes in Actinobacteria and are essential in notable pathogens such as Mycobacterium tuberculosis. The WhiA/B regulons and binding sites have been elucidated in Streptomyces venezuelae (Sven), where they coordinate to activate sporulation septation. However, how these factors cooperate at the molecular level is not understood. Here we present cryoelectron microscopy structures of Sven transcriptional regulatory complexes comprising RNA polymerase (RNAP) σ^A-holoenzyme and WhiA and WhiB, in complex with the WhiA/B target promoter sepX

SUBMITTER: Lilic M 

PROVIDER: S-EPMC10243135 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature