Fine-scale genomic tracking of Ross River virus using nanopore sequencing.

ABSTRACT:

Background

Ross River virus (RRV) is Australia's most common and widespread mosquito-transmitted arbovirus and is of significant public health concern. With increasing anthropogenic impacts on wildlife and mosquito populations, it is important that we understand how RRV circulates in its endemic hotspots to determine where public health efforts should be directed. Current surveillance methods are effective in locating the virus but do not provide data on the circulation of the virus and its strains within the environment. This study examined the ability to identify single nucleotide polymorphisms (SNPs) within the variable E2/E3 region by generating full-length haplotypes from a range of mosquito trap-derived samples.

Methods

A novel tiled primer amplification workflow for a

SUBMITTER: de Vries EM 

PROVIDER: S-EPMC10243270 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature