Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2.

ABSTRACT: Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.

SUBMITTER: Morales Vasquez D 

PROVIDER: S-EPMC10243362 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature