Hybrid-seq deciphers the complex transcriptional profile of the human BRCA1 DNA repair associated gene.

ABSTRACT: Breast Cancer Gene 1 (BRCA1) is a tumour suppressor protein that modulates multiple biological processes including genomic stability and DNA damage repair. Although the main BRCA1 protein is well characterized, further proteomics studies have already identified additional BRCA1 isoforms with lower molecular weights. However, the accurate nucleotide sequence determination of their corresponding mRNAs is still a barrier, mainly due to the increased mRNA length of BRCA1 (~5.5 kb) and the limitations of the already implemented sequencing approaches. In the present study, we designed and employed a multiplexed hybrid sequencing approach (Hybrid-seq), based on nanopore and semi-conductor sequencing, aiming to detect BRCA1 alternative transcripts in a panel of human cancer and non-c

SUBMITTER: Adamopoulos PG 

PROVIDER: S-EPMC10243389 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature