Preferential rabbit antibody responses to C-termini of NOTCH3 peptide immunogens.

ABSTRACT: Antibodies raised in peptide-immunized rabbits have been used in biological research for decades. Although there has been wide implementation of this approach, specific proteins are occasionally difficult to target for multiple reasons. One consideration that was noted in mice is that humoral responses may preferentially target the carboxyl terminus of the peptide sequence which is not present in the intact protein. To shed light on the frequency of preferential rabbit antibody responses to C-termini of peptide immunogens, we present our experience with generation of rabbit antibodies to human NOTCH3. A total of 23 antibodies were raised against 10 peptide sequences of human NOTCH3. Over 70% (16 of 23) of these polyclonal antibodies were determined to be C-terminal preferring: NOTCH3 pepti

SUBMITTER: Lee SJ 

PROVIDER: S-EPMC10244458 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature