Prominin-1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice.

ABSTRACT:

Purpose

Spermatogenesis is a complex process orchestrated by several essential genes. Prominin-1 (Prom1/PROM1) is a gene that is expressed in the testis but with a poorly understood role in spermatogenesis.

Methods

We used Prom1 knockout (Prom1 KO) mice to assess the role of Prom1 in spermatogenesis. To this end, we performed immunohistochemistry, immunofluorescence, western blotting, β-galactosidase staining, and apoptosis assay. Additionally, we analyzed the morphology of sperm and assessed litter sizes.

Results

We observed that PROM1 is localized to the dividing spermatocytes in seminiferous epithelial cells, sperm, and columnar epithelium in the epididymis. In the Prom1 KO testis, an aberrant increase in apoptotic cells an

SUBMITTER: Matsukuma H 

PROVIDER: S-EPMC10244806 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature