Measuring body temperature of freely moving mice under an optogenetics-induced long-term hypothermic state.
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ABSTRACT:
SUBMITTER: Takahashi TM
PROVIDER: S-EPMC10244883 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
Publications
Measuring body temperature of freely moving mice under an optogenetics-induced long-term hypothermic state.
Takahashi Tohru M TM Sakurai Takeshi T Hirano Arisa A
STAR protocols 20230531 2
We present a protocol for inducing a hibernation-like state in free-moving mice using optogenetics. We have recently developed an optogenetic technique utilizing modified Opsin4, which is activated by weak blue light, resulting in prolonged neuronal excitation. We describe a protocol that includes detailed instructions for virus injection, implantation of optic fibers and temperature transmitters, photostimulation, and real-time recording of body temperature in mice. This method is valuable for ...[more]
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