Pancreatic ductal adenocarcinoma ubiquitination profiling reveals specific prognostic and theranostic markers.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) has been widely studied at multiomics level. However, little is known about its specific ubiquitination, a major post-translational modification (PTM). As PTMs regulate the final function of any gene, we decided to establish the ubiquitination profiles of 60 PDAC.

Methods

We used specific proteomic tools to establish the ubiquitin dependent proteome (ubiquitinome) of frozen PDXs (Patients' derived xenographs). Then, we performed bioinformatics analysis to identify the possible associations of these ubiquitination profiles with tumour phenotype, patient survival and resistance to chemotherapies. Finally, we used proximity ligation assays (PLA) to detect and quantify the ubiquitination level of one identified marker.

Findin

SUBMITTER: El Kaoutari A 

PROVIDER: S-EPMC10244909 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature