Comparison of tumor-informed and tumor-naive sequencing assays for ctDNA detection in breast cancer.

ABSTRACT: Analysis of circulating tumor DNA (ctDNA) to monitor cancer dynamics and detect minimal residual disease has been an area of increasing interest. Multiple methods have been proposed but few studies have compared the performance of different approaches. Here, we compare detection of ctDNA in serial plasma samples from patients with breast cancer using different tumor-informed and tumor-naïve assays designed to detect structural variants (SVs), single nucleotide variants (SNVs), and/or somatic copy-number aberrations, by multiplex PCR, hybrid capture, and different depths of whole-genome sequencing. Our results demonstrate that the ctDNA dynamics and allele fractions (AFs) were highly concordant when analyzing the same patient samples using different assays. Tumor-informed assays showed the

SUBMITTER: Santonja A 

PROVIDER: S-EPMC10245040 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature