Dataset Information

Aging Affects the Efficacy of Platelet-Rich Plasma Treatment for Osteoarthritis.

ABSTRACT:

Objective

Despite the increased use of platelet-rich plasma (PRP) in the treatment of osteoarthritis (OA), whether and how age of the PRP donor affects therapeutic efficacy is unclear.

Design

In vitro, male osteoarthritic human chondrocytes were treated with PRP from young (18-35 years) or old (≥65 years) donors, and chondrogenic profile was evaluated using immunofluorescent staining for two markers of chondrogenicity, type II collagen and SOX-9. In vivo, we used a within-subjects design to compare Osteoarthritis Research Society International (OARSI) scores in aged mouse knee joints injected with PRP from young or old individuals.

Results

In vitro experiments revealed that PRP from young donors induced a more youthful chondrocyte phenotype, as evidenced by increased type II collagen (p = 0.033) and SOX-9 expression (p = 0.022). This benefit, however, was significantly blunted when cells were cultured with PRP from aged donors. Accordingly, in vivo studies revealed that animals treated with PRP from young donors displayed a significantly improved cartilage integrity when compared to knees injected with PRP from aged donors (p = 0.019).

Conclusion

Injection of PRP from a young individual induced a regenerative effect in aged cells and mice, whereas PRP from aged individuals showed no improvement chondrocyte health and cartilage integrity.

SUBMITTER: Chowdhary K

PROVIDER: S-EPMC10245083 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Publications

Aging Affects the Efficacy of Platelet-Rich Plasma Treatment for Osteoarthritis.

Chowdhary Kuntal K Sahu Amrita A Iijima Hirotaka H Shinde Sunita S Borg-Stein Joanne J Ambrosio Fabrisia F

American journal of physical medicine & rehabilitation 20221207 7

<h4>Objective</h4>Despite the increased use of platelet-rich plasma in the treatment of osteoarthritis, whether and how age of the platelet-rich plasma donor affects therapeutic efficacy is unclear.<h4>Design</h4>In vitro, male osteoarthritic human chondrocytes were treated with platelet-rich plasma from young (18-35 yrs) or old (≥65 yrs) donors, and the chondrogenic profile was evaluated using immunofluorescent staining for two markers of chondrogenicity, type II collagen and SOX-9. In vivo, we ...[more]

PMID: 36480365

Similar Datasets

Project description:BackgroundThis study aimed to evaluate the clinical efficacy of platelet-rich plasma (PRP) injection compared with hyaluronic acid (HA) injection for patients undergoing knee osteoarthritis.MethodsWe systematically searched electronic databases including PubMed, Embase, Web of Science, and the Cochrane Library on January 23, 2020 to identify relevant studies issued in English languages. The outcomes evaluating the efficacy of knee osteoarthritis (KOA) treatment were Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores (WOMAC pain, function, stiffness, and total scores) at 1, 3, 6, and 12 months; International Knee Documentation Committee (IKDC) scores, Lequesne Index score, Visual Analog Scale (VAS) scores, EQ-VAS scores, and KOOS scores. The pooled data were analyzed by Stata 12.0.ResultsA total of 20 RCTs were enrolled in the present meta-analysis. The pooled results demonstrated that platelet-rich plasma (PRP) injection reduced pain more effectively than hyaluronic acid (HA) injection at 6-month and 12-month follow-up evaluated by WOMAC pain scores and VAS scores. EQ-VAS in the patients treated with PRP injection was lower than that in patients with HA injection at 12 months. Moreover, the patients with PRP injection had a better function recovery than those with HA injection at 1-month, 3-month, 6-month, and 12-month follow-up, as evaluated by WOMAC function scores. WOMAC total scores showed significant difference at 6-month and 12-month follow-up. The IKDC scores indicated PRP injection was significantly more effective than HA injection at 3 months and 6 months. However, the Lequesne Index scores, KOOS scores, and adverse events did not show any significant difference between groups.ConclusionIntra-articular PRP injection appeared to be more efficacious than HA injection for the treatment of KOA in terms of short-term functional recovery. Moreover, PRP injection was superior to HA injection in terms of long-term pain relief and function improvement. In addition, PRP injection did not increase the risk of adverse events compared to HA injection.

Dataset Information

Aging Affects the Efficacy of Platelet-Rich Plasma Treatment for Osteoarthritis.

Objective

Design

Results

Conclusion

Publications

Aging Affects the Efficacy of Platelet-Rich Plasma Treatment for Osteoarthritis.

Similar Datasets

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