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Parameters optimization of Fe₃O₄ NPs synthesis by Tamarindus indica leaf extract possessing both peroxidase as well as excellent dye removal activity.

ABSTRACT: This study reports optimized conditions for the green synthesis of iron (II,III) oxide nanoparticles (Fe₃O₄ NPs) from Tamarindus indica (T. indica) leaf extract. The synthetic parameters like concentration of leaf extract, solvent system, buffer, electrolyte, pH, and time were optimized for Fe₃O₄ NPs synthesis. Fe₃O₄ NPs were obtained from the synthesis protocol by measuring size (80 ± 3 nm approx.), characteristics color changes, and an absorption peak between 270 nm and 280 nm using a UV-visible spectrophotometer, scanning electron microscope (SEM), and an energy dispersive X-ray spectrometer (EDS) study. Peroxidase activity was tested with 3,3,5,5-Tetramethylbenzidine (TMB) oxidation in the presence of hydrogen peroxide and dye removal activity was tested with malachite green (MG). The results indicated that the successful synthesis of Fe₃O₄ nanoparticles using aqueous leaf extract of T. indica is a practical alternative for biomedical applications due to its potent peroxidase activity and high dye removal capacity (about 93% with UV light and 55% with room light).

SUBMITTER: Akanda MR

PROVIDER: S-EPMC10245268 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Parameters optimization of Fe3O4 NPs synthesis by Tamarindus indica leaf extract possessing both peroxidase as well as excellent dye removal activity.

Akanda Md Rajibul MR Al-Amin Md M Mele Masrifa Akter MA Shuva Zunayed Mahmud ZM Hossain Md Billal MB Islam Taohedul T Hasan Md Mehedi MM Ema Umme Habiba UH

Heliyon 20230527 6

This study reports optimized conditions for the green synthesis of iron (II,III) oxide nanoparticles (Fe3O4 NPs) from Tamarindus indica (T. indica) leaf extract. The synthetic parameters like concentration of leaf extract, solvent system, buffer, electrolyte, pH, and time were optimized for Fe3O4 NPs synthesis. Fe3O4 NPs were obtained from the synthesis protocol by measuring size (80 ± 3 nm approx.), characteristics color c ...[more]

PMID: 37292316

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Project description:The leaf extract of Tamarindus indica L. (T. indica) had been reported to possess high phenolic content and showed high antioxidant activities. In this study, the effects of the antioxidant-rich leaf extract of the T. indica on lipid peroxidation, antioxidant enzyme activities, H2O2-induced ROS production and gene expression patterns were investigated in liver HepG2 cells. Lipid peroxidation and ROS production were inhibited and the activity of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase was enhanced when the cells were treated with the antioxidant-rich leaf extract. cDNA microarray analysis revealed that 207 genes were significantly regulated by at least 1.5-fold (p < 0.05) in cells treated with the antioxidant-rich leaf extract. The expression of KNG1, SERPINC1, SERPIND1, SERPINE1, FGG, FGA, MVK, DHCR24, CYP24A1, ALDH6A1, EPHX1 and LEAP2 were amongst the highly regulated. When the significantly regulated genes were analyzed using Ingenuity Pathway Analysis software, "Lipid Metabolism, Small Molecule Biochemistry, Hematological Disease" was the top biological network affected by the leaf extract, with a score of 36. The top predicted canonical pathway affected by the leaf extract was the coagulation system (P < 2.80 × 10(-6)) followed by the superpathway of cholesterol biosynthesis (P < 2.17 × 10(-4)), intrinsic prothrombin pathway (P < 2.92 × 10(-4)), Immune Protection/Antimicrobial Response (P < 2.28 × 10(-3)) and xenobiotic metabolism signaling (P < 2.41 × 10(-3)). The antioxidant-rich leaf extract of T. indica also altered the expression of proteins that are involved in the Coagulation System and the Intrinsic Prothrombin Activation Pathway (KNG1, SERPINE1, FGG), Superpathway of Cholesterol Biosynthesis (MVK), Immune protection/antimicrobial response (IFNGR1, LEAP2, ANXA3 and MX1) and Xenobiotic Metabolism Signaling (ALDH6A1, ADH6). In conclusion, the antioxidant-rich leaf extract of T. indica inhibited lipid peroxidation and ROS production, enhanced antioxidant enzyme activities and significantly regulated the expression of genes and proteins involved with consequential impact on the coagulation system, cholesterol biosynthesis, xenobiotic metabolism signaling and antimicrobial response.

Dataset Information

Parameters optimization of Fe₃O₄ NPs synthesis by Tamarindus indica leaf extract possessing both peroxidase as well as excellent dye removal activity.

Publications

Parameters optimization of Fe<sub>3</sub>O<sub>4</sub> NPs synthesis by <i>Tamarindus indica</i> leaf extract possessing both peroxidase as well as excellent dye removal activity.

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