Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis.

ABSTRACT:

Background

Silica-induced pulmonary fibrosis (silicosis) is a diffuse interstitial fibrotic disease characterized by the massive deposition of extracellular matrix in lung tissue. Fibroblast to myofibroblast differentiation is crucial for the disease progression. Inhibiting myofibroblast differentiation may be an effective way for pulmonary fibrosis treatment.

Methods

The experiments were conducted in TGF-β treated human lung fibroblasts to induce myofibroblast differentiation in vitro and silica treated mice to induce pulmonary fibrosis in vivo.

Results

By quantitative mass spectrometry, we revealed that proteins involved in mitochondrial folate metabolism were specifically upregulated during myofibroblast differentiation following TGF-β stimulation. The expression l

SUBMITTER: Qu Y 

PROVIDER: S-EPMC10245413 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature