Defining distinct RNA-protein interactomes of SARS-CoV-2 genomic and subgenomic RNAs.

ABSTRACT: Host RNA binding proteins recognize viral RNA and play key roles in virus replication and antiviral defense mechanisms. SARS-CoV-2 generates a series of tiered subgenomic RNAs (sgRNAs), each encoding distinct viral protein(s) that regulate different aspects of viral replication. Here, for the first time, we demonstrate the successful isolation of SARS-CoV-2 genomic RNA and three distinct sgRNAs (N, S, and ORF8) from a single population of infected cells and characterize their protein interactomes. Over 500 protein interactors (including 260 previously unknown) were identified as associated with one or more target RNA at either of two time points. These included protein interactors unique to a single RNA pool and others present in multiple pools, highlighting our ability to discriminate bet

SUBMITTER: Whitworth IT 

PROVIDER: S-EPMC10245570 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature