Infant and Adult Human Intestinal Enteroids are Morphologically and Functionally Distinct.

ABSTRACT:

Background & aims

Human intestinal enteroids (HIEs) are gaining recognition as physiologically relevant models of the intestinal epithelium. While HIEs from adults are used extensively in biomedical research, few studies have used HIEs from infants. Considering the dramatic developmental changes that occur during infancy, it is important to establish models that represent infant intestinal characteristics and physiological responses.

Methods

We established jejunal HIEs from infant surgical samples and performed comparisons to jejunal HIEs from adults using RNA sequencing (RNA-Seq) and morphologic analyses. We validated differences in key pathways through functional studies and determined if these cultures recapitulate known features of the infant intestinal epithelium.

Re

SUBMITTER: Adeniyi-Ipadeola GO 

PROVIDER: S-EPMC10245709 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature