In utero pulse injection of isotopic amino acids quantifies protein turnover rates during murine fetal development.

ABSTRACT: Protein translational control is highly regulated step in the gene expression program during mammalian development that is critical for ensuring that the fetus develops correctly and that all of the necessary organs and tissues are formed and functional. Defects in protein expression during fetal development can lead to severe developmental abnormalities or premature death. Currently, quantitative techniques to monitor protein synthesis rates in a developing fetus (in utero) are limited. Here, we developed a novel in utero stable isotope labeling approach to quantify tissue-specific protein dynamics of the nascent proteome during mouse fetal development. Fetuses of pregnant C57BL/6J mice were injected with isotopically labeled lysine (Lys8) and arginine (Arg10) via the vitell

SUBMITTER: Baeza J 

PROVIDER: S-EPMC10245746 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature