CRISPR-based engineering of RNA viruses.

ABSTRACT: CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a new recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

One-sentence summary

Programmable CRISPR RNA-guided ribonucleases enable recombinant RNA technology.

SUBMITTER: Nemudryi A 

PROVIDER: S-EPMC10245796 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature