Caenorhabditis elegans germ granules are present in distinct configurations that differentially associate with RNAi-targeted RNAs.

ABSTRACT: RNA silencing pathways are complex, highly conserved, and perform widespread, critical regulatory roles. In C. elegans germlines, RNA surveillance occurs through a series of perinuclear germ granule compartments-P granules, Z granules, SIMR foci, and Mutator foci-multiple of which form via phase separation and exhibit liquid-like properties. The functions of individual proteins within germ granules are well-studied, but the spatial organization, physical interaction, and coordination of biomolecule exchange between compartments within germ granule "nuage" is less understood. Here we find that key proteins are sufficient for compartment separation, and that the boundary between compartments can be reestablished after perturbation. Using super-resolution microscopy, we discover

SUBMITTER: Uebel CJ 

PROVIDER: S-EPMC10246010 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature