Development of Cas13a-based Assays for Neisseria gonorrhoeae Detection and Gyrase A Determination.

ABSTRACT:

Background

Neisseria gonorrhoeae is one of the most common bacterial sexually transmitted infections. The emergence of antimicrobial-resistant N. gonorrhoeae is an urgent public health threat. Currently, diagnosis of N. gonorrhoeae infection requires expensive laboratory infrastructure, while antimicrobial susceptibility determination requires bacterial culture, both of which are infeasible in low-resource areas where prevalence is highest. Recent advances in molecular diagnostics, such as Specific High-sensitivity Enzymatic Reporter unLOCKing (SHERLOCK) using CRISPR-Cas13a and isothermal amplification, have the potential to provide low-cost detection of pathogen and antimicrobial resistance.

Methods and results

We designed and optimized RNA guides and p

SUBMITTER: Allan-Blitz LT 

PROVIDER: S-EPMC10246164 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature